Use of hydrolytic and oxidative enzymes to dissolve biofilm...

A - Human Necessities – 61 – K

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A61K 38/47 (2006.01) A61K 38/54 (2006.01) A61P 31/04 (2006.01)

Patent

CA 2692791

A composition for removal of biofilm in the airway passage is useful for the treatment of infections such as pneumonia caused by Mycoplasma pneumoniae. In general, the composition comprises: (1 ) a quantity of at least one enzyme that catalyzes the hydrolysis of a bond that connects two monosaccharides in a polysaccharide or that connects a monosaccharide with a protein molecule in a glycoprotein sufficient to break down biofilm in the airway; and (2) a pharmaceutically acceptable carrier suitable for administration into the airway The composition can further include ingredients such as a steroid, lysozyme, lactoferrin, or a peroxidase; if a peroxidase is included, the composition can further include an oxidase to generate peroxide as well as a substrate for the oxidase. The composition can be used in methods for treatment of an infection based on the ability of the composition to dissolve biofilm in the airway.

L'invention concerne une composition pour l'élimination d'un biofilm dans les voies aériennes utile pour le traitement d'infections telles que la pneumonie provoquée par Mycoplasma pneumoniae. En général, la composition comprend : (1) une quantité d'au moins une enzyme catalysant l'hydrolyse d'une liaison qui relie deux monosaccharides dans un polysaccharide ou qui relie un monosaccharide à une molécule de protéine dans une glycoprotéine suffisante pour décomposer un biofilm dans les voies aériennes ; et (2) un support pharmaceutiquement acceptable approprié pour une administration dans les voies aériennes. La composition peut en outre comprendre des ingrédients tels qu'un stéroïde, un lysozyme, une lactoferrine, ou une peroxydase ; si une peroxydase est incluse, la composition peut en outre comprendre une oxydase pour générer un peroxyde ainsi qu'un substrat pour l'oxydase. La composition peut être utilisée dans des procédés de traitement d'une infection basés sur la capacité de la composition à dissoudre le biofilm dans les voies aériennes.

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