Use of igf-bp for refolding of igf

Details Use of igf-bp for refolding of igf Use of igf-bp for refolding of igf

C - Chemistry, Metallurgy

07

K

C07K 14/65 (2006.01)

Patent

CA 2176599

The invention relates to the use of Insulin-like growth factor binding protein (IGF-BP) for refolding of Insulin-like growth factor (IGF).The binding protein is preferably any of IGF-BP-1,IGF-BP-2,IGF-BP-3,IGP-BP-4,IGF-BP-5 and IGF-BP-6 and the Insulin-like growth factor is preferably IGF-I. It also relates to the process for the production of biologically active, native IGF-I or IGF-II, characterized in that IGF-I or IGF-II in a reduced or misfolded form is subjected to treatment with IGF-BP.

L'invention concerne l'utilisation de la protéine de liaison du facteur de croissance de substances apparentées à l'insuline (IGF-BP) pour le repliement de ce facteur de croissance de substances apparentées à l'insuline (IGF). La protéine de liaison est, de préférence, l'IGF-BP-1, IGF-BP-2, IGF-BP-3, IGF-BP-4, IGF-BP-5 et IGF-BP-6 et le facteur de croissance de substances apparentées à l'linsuline est, de préférence, l'IGF-I. L'invention a également pour objet le procédé permettant de produire l'IGF-I ou IGF-II biologiquement actif, natif, caractérisé en ce que l'IGF-I ou l'IGF-II sous une forme réduite ou présentant un défaut de repliement est traité par l'IGF-BP.

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