Use of inhibitors of color generation in chromogenic assays

Details Use of inhibitors of color generation in chromogenic assays Use of inhibitors of color generation in chromogenic assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/26 (2006.01) C12Q 1/28 (2006.01)

Patent

CA 2082756

ABSTRACT OF THE DISCLOSURE Disclosed is a novel diagnostic reagent system for the detection of hydroperoxides or substances which react with peroxidatively active substances resulting in the liberation of hydroperoxides which reagent system comprises a chromogenic oxidation indicator and a peroxidase or a peroxidatively active substance, together with an inhibitor of color generation selected from the group of 1,4-disubstituted semicarbazides, thiosemicarbazides and 1,5-disubstituted carbazides as inhibitors of color generation. By inhibiting the generation of color formed by oxidation of the chromogenic indicator, the indicator's usefulness in discriminating between varying concentrations of analyte is enhanced. MS #1679

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