Use of intramolecularly, covalently cross-linked proteins as...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/12 (2006.01) C07K 14/155 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2427687

The invention concerns the use of intramolecularly, covalently cross-linked proteins, the use of covalently cross-linked reverse transcriptase from HIV as immunological binding partners in immunoassays. It also concerns immunological test procedures for detecting an analyte in a sample in which intramolecularly, covalently cross--linked proteins are used as binding partners and concerns intramolecularly, covalently cross-linked reverse transcriptase from HIV and a method for producing this reverse transcriptase.

L'invention concerne l'utilisation de protéines à réticulation covalente intramoléculaires, l'utilisation de Transcriptase inverse à réticulation covalente de HIV comme partie liante immunologique dans immuno-essais, des procédés de test immunologique permettant de détecter un analyte dans un prélèvement, des protéines à réticulation moléculaire intramoléculaires étant utilisées comme parties liantes. L'invention concerne également la transcriptase inverse à réticulation covalente intramoléculaire de HIV ainsi qu'un procédé de production de cette transcriptase inverse.

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