Use of isogenic drug-resistant cell lines to determine the...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) A61K 47/42 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2546622

The present invention provides isogenic cell lines and uses said isogenic cell lines in a method for determining a sequence to administer multiple types of chemotherapeutic drugs for killing cancerous cells to reduce the induction of drug cross-resistance in a patient. The methods also involves screening drug candidates to select a lead anticancer drug from amongst a plurality of candidate drugs, the lead having a reduced capacity to induce cross resistance in a patient against one or more known anticancer drugs, and all of the drugs having the ability to kill cancerous cells of the same selected tumour type. Moreover, the methods involve determining a sequence to administer multiple types of cytotoxic drugs for killing undesired cells to reduce the indication of drug cross-~resistance in the cells.

Méthode servant à déterminer une séquence d'administration de types multiples de médicaments chimiothérapeutiques servant à détruire des cellules cancéreuses, dans le but de limiter l'induction d'une résistance croisée aux médicaments chez le patient. Cette méthode consiste également à cribler les médicaments candidats afin de sélectionner un médicament anticancer approprié parmi une pluralité de médicaments candidats, ce médicament présentant une capacité limitée d'induction de résistance croisée du patient contre un ou plusieurs médicaments anticancer connus, la totalité de ces médicaments possédant la capacité de détruire les cellules cancéreuses du même type de tumeur sélectionné. Cette méthode consiste, de plus, à déterminer une séquence d'administration de types multiples de médicaments cytotoxiques servant à détruire les cellules indésirables afin de limiter l'induction d'une résistance croisée au médicament dans ces cellules.

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