Use of magnetic material to direct isolation of compounds...

Details Use of magnetic material to direct isolation of compounds... Use of magnetic material to direct isolation of compounds...

G - Physics

01

N

G01N 33/543 (2006.01) B01D 15/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2575446

Methods for isolating a compound from a multipart, typically biological sample. The methods use at least one paramagnetic particle having an associated electronic charge to bind compounds with the opposite charge to form a particle/compound complex. Alternatively, the paramagnetic particles have a ligand or functional group with an affinity for a target compound to form a particle/compound complex. The complex can be immobilized by applying a magnetic field to the particle/protein complex. The sample may be further processed to obtain a protein sample in a more pure form or a sample depleted of select compounds.

L'invention concerne des procédés d'isolement d'un composé d'un échantillon biologique typique constitué de plusieurs parties. Ces procédés utilisent au moins une particule paramagnétique ayant une charge électronique associée pour lier les composés à la charge opposée afin de former un complexe particule/composé. En variante, les particules paramagnétiques ont un ligand ou un groupe fonctionnel ayant une affinité pour un composé cible afin de former un complexe particule/composé. Le complexe peut être immobilisé par l'application d'un champ magnétique au complexe particule/protéine. L'échantillon peut être davantage traité pour obtenir un échantillon protéique d'une forme plus pure ou un échantillon appauvri en composés sélectionnés.

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