Use of magnetic material to fractionate samples

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 1/14 (2006.01)

Patent

CA 2575784

A method useful for the reversible binding of a protein molecule in a biological sample. The method uses paramagnetic particles having an associated electronic charge to bind proteins with the opposite charge to form a particle/protein complex. The complex can be immobilized to a container wall by applying a magnetic field to the particle/protein complex. The sample may be further processed to obtain a protein sample in a more pure form or a sample depleted of select proteins.

La présente invention concerne un procédé permettant une liaison réversible de la molécule de protéine dans un échantillon biologique. Ce procédé se sert des particules paradigmatiques comprenant une charge électronique associée pour lier les protéines avec une charge opposée pour former un complexe de particule protéine. Le complexe peut être immobilisé dans une paroi de contenant en appliquant un champ magnétique sur le complexe de particule/protéine. L~échantillon peut en outre être traité pour obtenir un échantillon de protéine sous une forme plus pire ou un échantillon pauvre en protéines sélectionnées.

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