Use of malate dehydrogenase for nadh regeneration

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 13/04 (2006.01) C12N 9/04 (2006.01) C12N 9/06 (2006.01) C12N 15/53 (2006.01) C12P 7/04 (2006.01) C12P 7/40 (2006.01) C12P 13/06 (2006.01) C12P 13/22 (2006.01) C12P 19/36 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2497499

The present invention relates to a process for the preparation of enantiomerically enriched organic compounds. In particular, the present invention relates to an enzymatically operating process, in which, in a coupled enzymatic reaction system, NAD(P)H is consumed by one enzyme for the preparation of the organic compound and the NAD(P)H is simultaneously regenerated by a second enzyme system. A reaction system which operates according to the invention in this manner and an advantageous whole cell catalyst are also proposed.

Cette invention, qui a trait à un procédé permettant de préparer des composés organiques énantiomériquement enrichis, concerne notamment un mode opératoire par voie enzymatique dans le cadre duquel, dans un système couplé de réaction enzymatique, NAD (P) H est digéré par une enzyme aux fins de la préparation dudit composé organique et simultanément régénéré par un second système de réaction. L'invention porte également sur un système de réaction agissant comme décrit et sur un avantageux catalyseur de cellule totale.

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