Use of mariner transposan in the production of transgenic...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2300972

A method for the preparation of a transgenic animal embryo comprising the step of introducing a mariner-like element (MLE) containing a transgene into an animal embryo cell, optionally including the step of introduction of exogenous transposase protein, or a DNA or RNA sequence encoding a transposase. The resulting embryo may be used to generate further embryos or be allowed to develop into an animal. The invention is useful in introducing foreign DNA into selected animals.

L'invention concerne un procédé pour créer un embryon d'animal transgénique. Ce procédé consiste à introduire, dans une cellule embryonnaire de l'animal, un élément semblable au Mariner (MLA) qui comprend un transgène, et éventuellement à introduire une protéine transposase exogène ou une séquence d'ADN ou d'ARN codant pour une transposase. On peut utiliser l'embryon qui résulte de cette opération pour produire de nouveaux embryons ou pour lui permettre de se développer et donner un animal. L'invention peut servir à introduire des ADN étrangers dans des animaux sélectionnés.

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