Use of multiple recombination sites with unique specificity...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C07K 14/47 (2006.01) C12N 9/00 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/64 (2006.01) C12N 15/66 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2392753

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.

La présente invention concerne des compositions et des procédés permettant le clonage de recombinaison. Ces compositions comprennent des vecteurs présentant de multiples sites de recombinaison avec une spécificité unique. Ces procédés permettent le clonage simultané de deux ou plusieurs molécules d'acide nucléique différentes. Dans certains modes de réalisation, les molécules sont soudées ensemble tandis que dans d'autres modes de réalisation, elles sont insérées dans des sites distincts d'un vecteur. L'invention traite également de la liaison ou de l'assemblage par recombinaison d'un certain nombre de molécules et/ou de composés (par exemple, de produits chimiques, de médicaments, de protéines ou de peptides, de lipides, d'acides nucléiques, d'hydrates de carbone, etc..) qui peuvent être semblables ou différents. Ces molécules et/ou ces composés ou les combinaisons de ces molécules et/ou de ces composés peuvent également se lier par recombinaison à diverses structures ou supports selon la présente invention.

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