Use of neurite localized mrnas for medical diagnosis and...

Details Use of neurite localized mrnas for medical diagnosis and... Use of neurite localized mrnas for medical diagnosis and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2204433

A method of identifying neurite cDNA clones by determining and comparing mRNA expression in selected neurites is provided. cDNA clones identified by this method are also provided. In addition, methods of profiling mRNA expression and diagnosing and treating conditions associated with a pattern of mRNA expression by determining an mRNA expression profile are provided. The figure illustrates a differential display pattern obtained in neurites using the oligonucleotide OP-5 as the 3' primer.

Un procédé d'identification de clones d'ADNc dans les neurites consiste à déterminer et à comparer l'expression d'ARNm dans des neurites sélectionnés. Des clones d'ADNc identifiés par ce procédé sont également décrits. De plus, l'invention se rapporte à des procédés destinés à établir le profil d'expression de l'ARNm et à diagnostiquer et traiter des états associés au modèle de l'expression de l'ARNm par détermination d'un profil d'expression de l'ARNm. La figure 1A présente un modèle de visualisation différentielle obtenu dans les neurites à l'aide de l'oligonucléotide OP-5 utilisé comme amorce 3'.

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