Use of nucleic acids bound to carrier macromolecules

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2273078

A nucleic acid primer is bound to a soluble carrier macromolecule, a multitude of primer molecules thus being carried by each carrier macromolecule, and hybridisation of the primer to a template followed by extension of the primer to replicate the template in complementary form is carried out as part of a PCR procedure or other amplification, or to form an extended primer of greater hybridisation affinity. A second primer used in the amplification may also be bound to a carrier macromolecule.

Une amorce d'acide nucléique est liée à une macromolécule porteuse soluble, une multitude de molécules d'amorce étant ainsi transportée par chaque macromolécule porteuse. L'hybridation de l'amorce sur une matrice suivie de l'allongement de l'amorce pour répliquer la matrice sous une forme complémentaire est effectuée en tant que partie d'une procédure ACP ou d'une autre amplification, ou pour former une amorce allongée ayant une meilleure affinité d'hybridation. Une seconde amorce utilisée dans l'amplification peut également être liée à une macromolécule porteuse.

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