Use of nucleic acids containing unmethylated cpg...

A - Human Necessities – 61 – K

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Details

A61K 39/39 (2006.01) A61K 39/29 (2006.01)

Patent

CA 2283557

The present invention is based on the finding that nucleic acids containing at least one unmethylated cytosine-guanine (CpG) dinucleotide affect immune responses in a subject. These nucleic acids containing at least one unmethylated cytosine-guanine (CpG) dinucleotide can be used to induce an immune response in a subject. The method includes administering to the subject a therapeutically effective amount of nucleic acid encoding an antigenic polypeptide, and a therapeutically effective amount of an oligonucleotide containing at least one unmethylated CpG dinucleotide. The invention also provides a method for treating a subject having or at risk of having viral- mediated disorder, comprising administering to the subject a therapeutically effective amount of a nucleic acid encoding an antigenic polypeptide and an effective amount of an oligonucleotide containing at least one unmethylated CpG dinucleotide.

Cette invention est fondée sur la découverte que des acides nucléiques contenant au moins un dinucléotide de cytosine-guanine (CpG) non méthylé affectent les réponses immunes chez un sujet. Ces acides nucléiques contenant au moins un dinucléotide de cytosine-guanine (CpG) non méthylé peuvent être utilisés pour induire une réponse immune chez un sujet. Le procédé consiste à administrer au sujet une dose thérapeutique efficace d'acide nucléique codant un polypeptide antigénique et une dose thérapeutique efficace d'un oligonucléotide contenant au moins un dinucléotide CpG non méthylé. Cette invention concerne également un procédé de traitement d'un sujet présentant déjà un dérèglement d'origine virale ou susceptible de le présenter, ce procédé consistant à administrer au sujet une dose thérapeutique efficace d'un acide nucléique codant un polypeptide antigénique et une dose efficace d'un oligonucléotide contenant au moins un dinucléotide CpG non méthylé.

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