Use of organic buffers to reduce dehydroascorbic acid...

Details Use of organic buffers to reduce dehydroascorbic acid... Use of organic buffers to reduce dehydroascorbic acid...

G - Physics

01

N

150/15.1, 150/16

G01N 33/50 (2006.01) C12Q 1/00 (2006.01) C12Q 1/04 (2006.01) C12Q 1/28 (2006.01)

Patent

CA 1290666

USE OF ORGANIC BUFFERS TO REDUCE DEHYDROASCORBIC ACID INTERFERENCE IN ANALYTICAL METHODS Abstract of the Disclosure The interference of dehydroascorbic acid, an oxidation product of ascorbic acid, in analytical methods is reduced with the use of certain nitrogen- containing organic buffers. Dehydroascorbic acid tends to cause premature reduction of reducible com- pounds which are used to provide detectable species in the presence of an analyte. These organic buf- fers, as opposed to inorganic buffers, prevent pre- mature reduction of the reducible compounds. An ana- lytical method can be carried out in solution or in a dry analytical element.

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