Use of oxalate deficient aspergillus niger strains for...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/14 (2006.01) C12N 15/80 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2513833

The invention relates to oxalate deficient A. niger strains for the production of a given enzyme, wherein the oxalate deficient strain produces at least the same amount of enzyme as the wild type strain it originates from under the same culture conditions. Preferably, the oxalate deficient A. niger strain produces more enzyme than the wild type strain it originates from under the same culture conditions. More preferably, the oxalate deficient A. niger strain is such that when the strain has been transformed with an expression construct comprising a gene coding for an enzyme, said strain produces at least the amount of the enzyme the wild type strain it originates from would produce under the same culture conditions, when the wild type strain has also been transformed with the same expression construct as the oxalate deficient strain. The invention also relates to method for obtaining such oxalate deficient A. niger strain. The present invention further relates to method for producing an enzyme, wherein an oxalate deficient A. niger strain that produces at least the same amount of enzyme as the wild type strain it originates from under the same culture conditions is used.

L'invention porte sur des souches de A. niger déficientes en oxalate utilisées pour la production d'une enzyme particulière, et qui produisent au moins la même quantité sinon plus de l'enzyme que la souche sauvage dont on les a tiré, dans les mêmes conditions de culture. Encore mieux, la souche de A. niger déficiente en oxalate est telle que quand on la transforme à l'aide d'un gène hybride du type codant pour une enzyme, elle produit au moins la même quantité d'enzyme que la souche sauvage dont on l'a tirée quand cette dernière a été traitée avec le même gène hybride, dans les mêmes conditions de culture. L'invention porte en outre sur le procédé d'obtention une telle souche de A. niger déficiente en oxalate. L'invention porte également sur un procédé de production d'une enzyme selon lequel une souche de A. niger déficiente en oxalate produit au moins la même quantité de l'enzyme que la souche sauvage dont on l'a tiré, et dans les mêmes conditions de culture.

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