Use of oxidised lipoproteins for differentiation of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0784 (2010.01) A61K 38/16 (2006.01) A61K 39/00 (2006.01) A61P 31/00 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2452630

The invention concerns the use of at least a fraction of oxidised (VLDL oxidised and/or IDL oxidised and/or LDL oxidised) lipoproteins for differentiating precursor cells into mature dendritic cells. The precursor cells are preferably monocytes. The invention also concerns a method for differentiating precursor cells into mature dendritic cells which consists in placing the precursor cells in a medium suitable for their differentiation and adding to said medium, in a predetermined amount, at least a fraction of oxidised lipoproteins selected among VLDL oxidised or IDL oxidised n or LDL oxidised lipoproteins. The precursor cells are preferably monocytes.

La présente invention concerne l'utilisation d'au moins une fraction de lipoprotéines oxydées (VLDL oxydées et/ou les IDL oxydées et/ou LDL oxydées) pour obtenir la différenciation de cellules précurseur en cellules dendritriques matures. De préférence, les cellules précurseur sont des monocytes. La présente invention concerne également un procédé pour la différenciation de cellules précurseur en cellules dendritriques matures selon lequel on dispose de cellules précurseur dans un milieu approprié permettant leur différenciation et on additionne audit milieu, en une quantité prédéterminée, au moins une fraction de lipoprotéines oxydées choisies parmi les VLDL oxydées ou les IDL oxydées ou LDL oxydées. De préférence, les cellules précurseur sont des monocytes.

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