Use of pectinolytic enzymes for the treatment of fruit and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/24 (2006.01)

Patent

CA 2724200

The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.

La présente invention concerne lutilisation dune ou de plusieurs enzymes pectinolytiques dans le traitement de purée de fruit ou de légume. Linvention porte également sur un procédé de traitement enzymatique de purée de fruit ou de légume comprenant létape consistant à ajouter une ou plusieurs enzymes pectinolytiques. Au moins une enzyme pectinolytique est obtenue à partir de Trichoderma reesei. Linvention concerne également un procédé de préparation dun jus de fruit ou de légume comprenant ledit procédé de traitement enzymatique de purée de fruit et de légume. En outre, linvention porte sur des molécules dADN recombinantes codant pour un polypeptide qui présente une activité endo-polygalacturonase, un polypeptide qui présente une activité exo-polygalacturonase, un polypeptide qui présente une activité exo-rhamnogalacturonase et un polypeptide qui présente une activité xylogalacturonase.

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