Use of phenols and anilines to increase the rate of...

C - Chemistry – Metallurgy – 12 – Q

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167/45, 150/15.1

C12Q 1/28 (2006.01) G01N 33/52 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1281643

USE OF PHENOLS AND ANILINES TO INCREASE THE RATE OF PEROXIDASE CATALYZED OXIDATION OF LEUCO DYES Abstract of the Disclosure Certain phenols and anilines can be used as electron transfer agents to increase the rate of oxi- dation of leuco dyes by peroxidase. These phenols end anilines can react with hydrogen peroxide in the presence of peroxidase to provide intermediates which have higher oxidation potentials than the slowly oxi- dized substrates, e.g. the leuco dyes. These phenols and anilines can be used to advantage in both solu- tion and dry assays of various analytes. They are particularly useful for the determination of an immunologically reactive ligand in an immunoassay.

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