Use of porous membrane to support developing conifer somatic...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/00 (2006.01) A01H 4/00 (2006.01) A01H 7/00 (2006.01) C12M 1/00 (2006.01) C12N 5/04 (2006.01)

Patent

CA 2563841

The present invention provides methods for developing conifer cotyledonary somatic embryos. In some embodiments, the methods of the invention include the step of culturing conifer pre-cotyledonary somatic embryos on a porous membrane, that is at least intermittently contacted with liquid development medium, for a period of time sufficient to produce conifer, cotyledonary, somatic embryos from the pre- cotyledonary somatic embryos.

La présente invention donne des méthodes de développement des embryons somatiques cotylédons des conifères. Dans certaines versions, les méthodes de l'invention comprennent l'étape de la culture des embryons somatiques pré-cotylédons des conifères sur une membrane poreuse. Cette membrane est au minimum mise en contact par intermittence avec un milieu de développement liquide, pendant une temps suffisant pour produire des embryons somatiques cotylédons de conifères, à partir d'embryons somatiques pré-cotylédons de conifères.

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