Use of pyrylium and thiapyrylium compounds as biological stains

Details Use of pyrylium and thiapyrylium compounds as biological stains Use of pyrylium and thiapyrylium compounds as biological stains

G - Physics

01

N

150/5

G01N 33/52 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1194766

-0- USE OF PYRYLIUM AND THIAPYRYLIUM COMPOUNDS AS BIOLOGICAL STAINS Abstract of the Disclosure Disclosed herein is in improvement in method for distinguishing cells in a biological sample by staining with a dye, wherein the improve- ment comprises employing as the dye a compound of the formula Image wherein G is O or S; R1, R3, and Rs are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)- vinylene, and Image wherein R is hydrogen or alkyl; Z represents the elements necessary to complete a basic heterocyclic ring system of the type used in cyanine dyes; n is 0 or 1; R2 is hydrogen or, taken together with either R1 or R3, represents the elements needed to complete an aromatic or carbocyclic ring system; R4 is hydrogen or, taken together with either R3 or R5, represents the elements needed to complete an aromatic or a carbocyclic ring system; and X- is an anion.

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