Use of spla2 activity for the diagnosis of a cardiovascular...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/44 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2659428

The present invention relates a method for determining an increased risk of mortality or of a cardiac and/or vascular event in a patient, comprising: - determining the sPLA2 activity of said patient as a first risk marker - determining at least the value of a second risk marker chosen among CRP level, IgM IC of apo B 100 level or IgM MDA-LDL level - determining the ratio (odds ratio) between the value of the sPLA2 activity and the value of the second risk marker and comparing it to a predetermined odds ratio, said odds ratio compared to said predetermined odds ratio being indicative of an increased risk of mortality or of a cardiac and /or vascular event. The present invention further relates a new micro method adaptation for automated fluorimetric measurement of serum secretory phospholipase A2.

La présente invention concerne un procédé destiné à déterminer un risque accru de mortalité ou d'événement cardiaque et/ou vasculaire chez un patient, consistant à : déterminer l'activité de la sPLA2 dudit patient en tant qu'un premier marqueur de risque ; déterminer au moins la valeur d'un deuxième marqueur de risque choisi parmi le niveau de CRP, le niveau des complexes IgM antigènes anti-apoprotéine B-100 native (apo B-100) ou le niveau d'IgM anti-MDA-LDL ; déterminer le rapport (rapport de cotes) entre la valeur de l'activité de la sPLA2 et la valeur du deuxième marqueur de risque, et comparer ce rapport à un rapport de cotes prédéfini, ledit rapport de cote comparé audit rapport de cotes prédéfini indiquant un risque accru de mortalité ou d'événement cardiaque et/ou vasculaire. La présente invention concerne en outre une nouvelle adaptation de méthode microscopique pour une mesure fluorimétrique automatisée de la phospholipase A2 sécrétoire dans le sérum sanguin.

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