Use of squaraine dyes to visualize protein during separations

Details Use of squaraine dyes to visualize protein during separations Use of squaraine dyes to visualize protein during separations

G - Physics

01

N

G01N 33/68 (2006.01) C09B 23/16 (2006.01) C09K 11/06 (2006.01) G01N 33/58 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2661663

Squaraine dyes are incorporated into a separation medium in which protein or polypeptide mixtures are separated, which medium also contains a detergent that forms a complex with the polypeptides or proteins. The dyes, upon excitation in the separation medium, exhibit a heretofore unrecognized selectivity in their fluorescent emissions by emitting a signal only when the dye molecules are associated with complexes of protein (or polypeptide) and detergent molecules, despite the additional presence of dyes in the bulk of the separation medium. The dyes are thus able to indicate the presence and locations of proteins or polypeptides in the separation medium without the need for removing unassociated dyes from the medium.

On a incorporé des colorants à la squaraine dans un milieu de séparation dans lequel on sépare des mélanges de protéines ou de polypeptides, ledit milieu contenant également un détergent qui forme un complexe avec les polypeptides ou les protéines. Les colorants, une fois excités dans le milieu de séparation, présentent une sélectivité jusqu'alors non constatée de leurs émissions fluorescentes et émettent un signal uniquement lorsque les molécules de colorant sont associées aux complexes de molécules de protéine (ou de polypeptide) et de détergent, malgré la présence additionnelle de colorants dans le volume global du milieu de séparation. Les colorants sont ainsi capables d'indiquer la présence et les positions des protéines ou des polypeptides dans le milieu de séparation sans qu'il soit nécessaire d'éliminer du milieu de séparation les colorants non associés.

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