Use of tomato e8-derived promoters to express...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/14 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2159101

The use of AdoMetase to reduce ethylene biosynthesis in plants is facilitated by the exploitation of the tissue and stage specific properties of the E8 promoter from tomato. Expression of AdoMetase is shown to be limited to the ripening tomato fruit The functional properties of several regions of the E8 promoter are described. The E8 promoter and variants described herein provide useful regulatable promoters for the expression of other genes as well as the AdoMetase gene.

L'utilisation d'AdoMétase pour réduire la biosynthèse de l'éthylène chez les plantes est rendue possible par l'exploitation des propriétés tissulaires et spécifiques aux étapes du promoteur de E8 de la tomate. L'expression de l'AdoMétase est limitée a la tomate mûrissante. Les propriétés fonctionnelles de plusieurs régions du promoteur de E8 sont décrites. Le promoteur de E8 et les variants décrits dans cette invention sont d'utiles promoteurs régulables employés pour exprimer d'autres gènes ainsi que le gène de l'AdoMétase.

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