G - Physics – 01 – N
Patent
G - Physics
01
N
167/45
G01N 33/554 (2006.01) A61K 39/44 (2006.01) G01N 33/53 (2006.01) G01N 33/78 (2006.01)
Patent
CA 1100869
ABSTRACT OF THE DISCLOSURE: The invention is concerned with a method for the determination of the concentration of tri-iodo thyronine in a sample. The method of the invention comprises extracting the tri-iodo thyronine of the sample being assayed by means of an insolubilized anti-T3 antibody derived from serum and tri-iodo thyronine; contacting the extracted tri-iodo thyronine in a column with a sensibilized enzyme consisting of an enzyme that is covalently bonded with tri-iodo thyronine and is derived from carbonic anhydrase and tri-iodo thyronine; hydrolyzing the effluent from the column with p-nitrophenyl acetate; and spectrophotometrically determining the concentration of tri-iodo thyronine by measuring the absorption of p-nitro- phenyl acetate hydrolyzed washings from the column in comparison with a standard. The invention enables one to dose tri-iodo thyronine in extremely small amount, in the order of the nano- gram and under.
298913
Baroncelli Vittorio
Renzi Pierluigi
Robic Robic & Associes/associates
Snamprogetti S.p.a.
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