Use of untranslated region of osmotin gene to enhance...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C07H 21/04 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/29 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2452131

The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5' and 3' untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

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