Use of virus-induced gene silencing (vigs) to down-regulate...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01)

Patent

CA 2717772

The present invention provides nucleic acid molecules and methods to down- regulate by virus- induced gene si-lencing (VIGS) vernalization genes in winter annuals, specifically the Flowering Locus C (FLC) gene in Brassica napus. Down-regulation of FLC allows winter annuals to flower without vernalization or with reduced vernalization. This, in turn, provides a shorter breeding cycling and the opportunity for enhanced genetic gain.

La présente invention porte sur des molécules d'acide nucléique et sur des procédés pour réguler de manière négative par silençage de gène induit par un virus (VIGS) des gènes de vernalisation dans des annuelles hivernales, spécifiquement le gène de Locus C de floraison (FLC) dans Brassica napus. Une régulation négative de FLC permet à des annuelles hivernales de fleurir sans vernalisation ou avec une vernalisation réduite. Ceci, à son tour, fournit un cycle de croissance plus court et l'opportunité d'un gain génétique amélioré.

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