Use of whole blood in pcr reactions

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2567620

A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.

L'invention concerne un procédé d'obtention d'amplification d'ADN d'une cible d'acide nucléique à partir d'un volume de sang entier consistant à effectuer une amplification d'ADN dans un mélange d'analyse PCR à l'aide d'une polymérase résistante présente dans le sang.

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