Use of zwitterions to mobilize isoelectrically focused...

B - Operations – Transporting – 01 – D

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B01D 57/02 (2006.01) C25B 7/00 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2075286

Ampholytic solute zones which have been stabilized by isoelectrical focusing in a separation medium such as a capillary are mobilized by the introduction of a relatively large quantity of zwitterion to one of the electrode chambers. When a voltage is then applied between the electrode chambers as in the focusing stage, the zwitterion migrates into the separation medium in accordance with the isoelectric point of the zwitterion. The voltage is maintained to cause continuous migration of the zwitterion into the capillary, thereby forming an expanding zone which displaces the focused zones formed in the focusing stage and causes then to travel axially through the capillary toward a detection point or out of the capillary for purposes of recovery.

Des zones de soluté ampholyte qui ont été stabilisées par concentration isoélectrique dans un milieu de séparation tel qu'un capillaire sont mobilisées par l'introduction d'une quantité relativement grande de zwitterions dans l'une des chambres d'électrode. Quand une tension est alors appliquée entre les chambres d'électrode, comme c'est le cas dans la phase de concentration, les zwitterions pénètrent dans le milieu de séparation selon leur point isoélectrique. Cette tension est maintenue pour entretenir une migration continue des zwitterions dans le capillaire et former ainsi une zone en expansion qui déplace les zones de concentration formées dans la phase de concentration et les oblige à avancer axialement dans le capillaire vers un point de détection ou à sortir du capillaire pour fins de récupération.

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