Uses of green fluorescent protein

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C12N 15/65 (2006.01) C12P 21/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2169298

This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green fluorescent protein operatively linked to a DNA sequence encoding the green fluorescent protein. This invention also provides living organisms which comprise the above-described cell. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green fluorescent protein; b) culturing the introduced cells under conditions permitting expression of the green fluorescent protein and the protein of interest; and c) selecting the cultured cells which express green fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green fluorescent protein.

L'invention concerne une cellule comprenant une molécule d'ADN présentant un élément régulateur en provenance d'un gène ne codant pas une protéine fluorescente verte liée de manière opératoire à une séquence d'ADN codant cette dernière. L'invention porte également sur des organismes vivants contenant ladite cellule ainsi que sur un procédé de sélection de cellules exprimant la protéine à étudier qui consiste à: a) introduire dans les cellules une molécule d'ADNI présentant une séquence d'ADN codant la protéine à étudier, et une molécule d'ADNII présentant une séquence d'ADN codant une protéine fluorescente verte; b) mettre en culture les cellules introduites dans des conditions permettant l'expression de la protéine fluorescente verte et la protéine à étudier. L'invention porte également sur les différentes utilisations d'une protéine fluorescente verte.

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