Uses of spatial configuration to modulate protein function

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/00 (2006.01) C12N 1/00 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2535902

This invention provides a set of methods for modulating protein spatial configuration. First, select the amino-acid codon for encoding the target protein according to host codon usage. Second, choose combinations which can modulate the spatial configuration and construct into different vectors which can transfect a series of hosts. Third, choose the vector promoter by monitoring a combination of base pairs after combining the code sequence of the promoter and the target protein. Finally, choose the appropriate expression host to express the target protein, refold and purify, measure the activity and spatial configuration.

Cette invention se rapporte à un ensemble de procédés de modulation de la configuration spatiale d'une protéine. Ces procédés consistent, premièrement, à sélectionner le codon d'acide aminé permettant de coder pour la protéine cible conformément à l'usage du codon chez l'hôte; deuxièmement, à choisir des combinaisons qui peuvent moduler la configuration spatiale et permettre l'élaboration de différents vecteurs pouvant transfecter une série d'hôtes; troisièmement, à choisir le promoteur vecteur au moyen du contrôle d'une combinaison de paires de bases après combinaison de la séquence codante du promoteur et de la protéine cible; et enfin, à choisir l'hôte d'expression approprié pour exprimer la protéine cible, à replier et purifier, et à mesurer l'activité et la configuration spatiale.

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