Using a reverse genetic engineering platform to produce...

Details Using a reverse genetic engineering platform to produce... Using a reverse genetic engineering platform to produce...

A - Human Necessities

61

K

A61K 39/145 (2006.01) A61P 31/16 (2006.01) C12N 15/10 (2006.01) C12P 21/02 (2006.01) C07K 14/11 (2006.01) C12N 7/00 (2006.01) C12N 15/44 (2006.01)

Patent

CA 2584894

The present invention relates to a preparation method of protein vaccines, and comprises the steps of: (a) providing at least one amino acid sequence of an epitope of a target antigen protein; (b) converting the amino acid sequence into a nucleic acid sequence and modifying the codons; (c) synthesising a plurality of primers of the modified nucleic acid sequence; (d) synthesising the modified nucleic acid sequence in vitro; (e) inserting the synthesized fragment of the modified nucleic acid sequence into a plasmid; (f) transforming the plasmid into a host cell to produce the modified nucleic acid encoded epitope peptide; and (g) collecting and purifying the produced peptide.

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