G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/571 (2006.01) C07K 16/12 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1262333
USING MONOCLONAL ANTIBODIES SPECIFIC FOR TREPONEMA BACTERIAL PATHOGENS ABSTRACT OF THE DISCLOSURE Murine anti-Treponema pallidum monoclonal antibodies were employed in the detection of low numbers of patho- genic treponemes. Monoclonal antibodies were used as a primary antibody source in a solid-phase immunoblot assay system. All monoclonal antibodies assayed were capable of detecting ca. 1.0 x 103 to 2.5 x 103 treponemes. Of 13 monoclonal antibodies examined, 3 were able to detect 103 virulent treponemes, and 1 of these antibodies was able to reveal the presence of as few as 500 organisms. Western blot analyses showed that all anti-T. pallidum monoclonal antibodies exhibiting high sensitivities for the detection of T. pallidum cells were directed against an abundant, 47,000-48,000 dalton surface-exposed antigen of the organism. With two possible exceptions, the monoclonal antibodies tested reacted specifically with T. pallidum, either purified or found within a high-contaminating tissue background, and not with Treponema phagedenis biotype Reiter, Haemophilus ducreyi, Neisseria gonorrhoeae, herpes simplex virus type 2, or normal rabbit testicular tissue.
501133
Kettman John R.
Norgard Michael V.
Meredith & Finlayson
The Board Of Regents Of The University Of Texas System
LandOfFree
Using monoclonal antibodies specific for treponema bacterial... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Using monoclonal antibodies specific for treponema bacterial..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Using monoclonal antibodies specific for treponema bacterial... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1235202