Vaccine for porcine reproductive and respiratory syndrome virus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/40 (2006.01) A61K 31/70 (2006.01) A61K 39/12 (2006.01) A61K 39/42 (2006.01) A61K 48/00 (2006.01) C07K 14/08 (2006.01) C07K 16/10 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2223608

The present invention provides a purified preparation containing, for example, a polynucleic acid encoding at least one polypeptide selected from the group consisting of proteins encoded by one or more open reading frames (ORF's) of an Iowa strain of porcine reproductive and respiratory syndrome virus (PRRSV), antigenic regions of such proteins which are at least 5 amino acids in length and which effectively protect a porcine host against a subsequent challenge with a PRRSV isolate, and combinations thereof in which amino acids non- essential for antigenicity may be conservatively substituted. The present invention also concerns a polypeptide encoded by such a polynucleic acid; a vaccine comprising an effective amount of such a polynucleic acid or protein; antibodies which specifically bind to such a polynucleic acid or protein; methods of producing the same; and methods of protecting a pig against a PRRSV, treating a pig infected by a PRRSV, and detecting a PRRSV using the same.

L'invention concerne une préparation purifiée contenant, par exemple, un acide polynucléique codant au moins un polypeptide choisi dans le groupe constitué de protéines codées par un ou plusieurs cadres de lecture ouverts dans une souche Iowa de virus du syndrome dysgénésique respiratoire porcin (PRRSV), de régions antigéniques de ces protéines d'au moins 5 acides aminés de longueur et protégeant efficacement un hôte porcin contre une confrontation ultérieure avec un isolat de PRRSV, et de combinaisons de celles-ci dans lesquelles des acides aminés non essentiels à l'antigénicité peuvent être substitués de manière conservatrice. L'invention concerne également un polypeptide codé par un tel acid polynucléique; un vaccin comprenant une dose efficace de cet acide polynucléique ou protéine; des anticorps se liant spécifiquement à cet acide polynucléique ou protéine; leurs procédés de production, ainsi que des procédés de protection d'un porc contre un PRRSV, de traitement d'un porc contaminé par un PRRSV et de détection d'un PRRSV à l'aide de ceux-ci.

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