Vaccine for the prevention of infections caused by...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/102 (2006.01) C12N 1/21 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2081950

ABSTRACT A vaccine composition for animal administration to elicit an enhanced immune response to challenge by P. haemolytica which produces leukotoxin proteins comprises a synergistic combination of vaccine components: 1. one or more biologically pure antigenic determinants of the leukotoxin protein, the one or more antigenic determinants each comprising an amino acid sequence of at least six amino acid residues selected from an amino acid sequence of Figure 1 and biological equivalents thereof; and 2. a bacterial-free culture supernatant derived from serum-free culture of P. haemolytica. An expression/secretion system for the leukotoxin protein is provided to enhance leukotoxin production.

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