Vaccines based on membrane bound proteins and process for...

A - Human Necessities – 61 – K

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167/129, 195/1.3

A61K 39/245 (2006.01) C07K 14/035 (2006.01) C12N 5/10 (2006.01) C12N 15/64 (2006.01) C12N 15/79 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1341181

The present invention relates to a vaccine comprising a polypeptide with antigenic determinants capable of raising neutralizing antibodies against a pathogen. The polypeptide may be functionally associated with a surface membrane of a recombinant, stable, continuous cell line capable of its production. Alternatively the polypeptide is formed first in functional association with a surface membrane of a recombinant, stable, continuous cell line capable of its production and then dissolved free from the membrane. The polypeptide may also be a truncated membrane-free derivative of a membrane-bound polypeptide. The derivative is formed by omission of a membrane-binding domain from the polypeptide allowing it to be secreted from a recombinant host cell system in which it has been produced. In particular the present invention provides for vaccines which are useful against HSV-1 and/or HSV-2 viruses thereby providing protective vaccines against occurrence of herpes infection and a reduction in frequency and severity of herpes infection recurrence in individuals already infected.

461897

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