Vaccines containing recombinant pilin against neisseria...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/095 (2006.01) C07K 14/22 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2325055

The pilE genes of each of Neisseria gonorrhoeae and Neisseria meningitidis are cloned and their corresponding recombinant pilin proteins are expressed. In addition, a chimeric pilE gene is constructed in which the region of the pilE gene of Neisseria meningitidis class I encoding the amino-terminal region of the pilin protein is replaced by the corresponding region of the pilE gene of Neisseria gonorrhoeae. The recombinant meningococcal chimeric class I pilin protein is expressed at higher levels than the pilin protein expressed by the full-length pilE gene of Neisseria meningitidis. Furthermore, a chimeric pilE gene is constructed in which the region of the pilE gene of Neisseria meningitidis class II encoding the carboxy-terminal region of the pilin protein is replaced by the corresponding region of the pilE gene of Neisseria gonorrhoeae. The recombinant pilin proteins are used in vaccines to protect against disease caused by Neisseria gonorrhoeae or Neisseria meningitidis.

Les gènes pilE de chacun de Neisseria gonorrhoeae et de Neisseria meningitidis sont clonés et leurs protéines piline de recombinaison correspondantes sont exprimées. En outre, un gène chimère pilE est construit dans lequel la région du gène pilE de Neisseria meningitidis de classe I codant la région N-terminale de la protéine piline est remplacée par la région correspondante du gène pilE de Neisseria gonorrhoeae. La protéine piline de classe I chimère méningococcique de recombinaison est exprimée à des taux supérieurs à la protéine piline exprimée par le gène pilE complet de Neisseria meningitidis. De surcroît un gène chimère pilE est construit dans lequel la région du gène pilE de Neisseria meningitidis de classe II codant la région C-terminale de la protéine piline est remplacée par la région correspondante du gène pilE de Neisseria gonorrhoeae. Les protéines piline de recombinaison sont utilisées dans des vaccins destinés à protéger contre les maladies provoquées par Neisseria gonorrhoeae ou Neisseria meningitidis.

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