Variable region sequences of il-31 monoclonal antibodies and...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/24 (2006.01) A61K 39/395 (2006.01) A61P 17/00 (2006.01) A61P 37/00 (2006.01) C12N 5/18 (2006.01)

Patent

CA 2660175

Novel compositions derived from antigen-binding sites of immunoglobulins having affinity for IL-31 are provided. The compositions exhibit immunological binding properties of antibody molecules capable of binding specifically to a human IL-31. CDR regions derived from same or different immunoglobulin moieties are provided. Also provided are single chain polypeptides wherein VH and VL domains are attached. The sFv molecules can include ancillary polypeptide moieties which can be bioactive, or which provide a site of attachment for other useful moieties. The compositions are useful in specific binding assays, affinity purification schemes, drug or toxin targeting, imaging, and genetic or immunological therapeutics for inflammatory diseases. The invention thus provides novel polypeptides, the DNAs encoding those polypeptides, expression cassettes comprising those DNAs, and methods of inducing the production of the polypeptides. The invention further provides the amino acid sequences of the variable regions of the monoclonal antibodies and use of these monoclonal antibody or antibody fragment in conjunction with an an human IgG4 Fc molecule.

L'invention concerne de nouvelles compositions provenant de sites de liaison antigène d'immunoglobulines ayant une affinité pour IL-31. Les compositions présentent des propriétés de liaison immunologiques de molécules d'anticorps pouvant se lier spécifiquement à l'IL-31 humain. Des zones CDR provenant de ces mêmes fragments d'immunoglobuline ou de fragments différents sont fournies. Sont également fournis des polypeptides à chaîne unique auxquels des domaines VH et VL sont attachés. Les molécules sFv peuvent inclure des fragments de polypeptide auxiliaire qui peuvent être bioactifs ou qui fournissent un site de fixation pour d'autres fragments utiles. Les compositions sont utiles dans des tests de liaison spécifiques, des schémas de purification d'affinité, des ciblages de médicament ou de toxine, une imagerie, et des thérapies géniques ou immunologiques pour des maladies inflammatoires. L'invention fournit ainsi de nouveaux polypeptides, les ADN codant ces polypeptides, des cassettes d'expression comprenant ces ADN, et des procédés pour induire la production des polypeptides. L'invention fournit, en outre, la séquence d'acides aminés des zones variables des anticorps monoclonaux et l'utilisation de ces anticorps ou de fragments d'anticorps monoclonaux en association avec une molécule humaine IgG4 Fc.

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