Variable volume assay device and method

Details Variable volume assay device and method Variable volume assay device and method

G - Physics

01

N

150/14, 167/44

G01N 33/53 (2006.01) B01L 3/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1291948

ABSTRACT OF THE DISCLOSURE An assay device for the detection of one or more compo- nents in a liquid sample, specifically antigens or anti- bodies in a biologically derived sample. The device defines a chamber of variable volume which is essentially sealed except for a membrane across an inlet port. The sample is placed on the exterior of the membrane. It is drawn to the chamber by suction created by decreasing the volume of the chamber. Liquid within the chamber is forced out of it under internal pressure created by decreasing the chamber volume. The chamber volume can be varied by twisting of a threadedly mounted piston in a body. In one embodiment, a sandwich assay for the de- tection of an antigen in a sample, the antigen is bound to immobilized antibody on a distinct region of the membrane and is also reacted with labeled antibody for detection. The sample is cycled through the membrane by varying the chamber volume.

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