Variable volume electroporation chamber and methods therefore

Details Variable volume electroporation chamber and methods therefore Variable volume electroporation chamber and methods therefore

C - Chemistry, Metallurgy

12

N

C12N 13/00 (2006.01) C12M 1/42 (2006.01)

Patent

CA 2631719

Disclosed is a chamber apparatus for electroporating in vitro relatively large volumes of a fluid medium carrying biological cells or vesicles wherein a reservoir for carrying said cells and vesicles is variable in its volume on demand and wherein the volume chosen is directly related to the volume of the sample to be electroporated. The apparatus has further embodiments wherein the chamber is disposable and can be operated either in isolation from a patient or connected thereto.

La présente invention concerne un appareil à enceinte d'électroporation in vitro de volumes relativement larges d'un milieu fluide transportant des cellules ou des vésicules biologiques, un réservoir pour transporter lesdites cellules et vésicules étant variable en volume à volonté, et le volume étant choisi en relation directe avec le volume de l'échantillon à soumettre à l'électroporation. D'autres modes de réalisation de l'appareil sont également prévus dans lesquels l'enceinte est jetable et peut fonctionner soit isolé d'un patient ou connecté à celui-ci.

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