C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/28 (2006.01) C12N 9/00 (2006.01) C12N 15/00 (2006.01)
Patent
CA 2726274
Alpha amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
La présente invention concerne des alpha amylases de Bacillus subtilis (AmyE), leurs variantes, des acides nucléiques les codant, et des cellules hôtes comprenant les acides nucléiques. Des procédés dutilisation dAmyE ou de ses variantes sont divulgués, comprenant la liquéfaction et/ou la saccharification damidon. De tels procédés peuvent donner des sucres utiles pour la production déthanol ou la production de sirop de maïs à haute teneur en fructose. Dans certains cas, les amylases peuvent être utilisées à un faible pH, en labsence de calcium et/ou en labsence de glucoamylase.
Cascao-Pereira Luis
Cuevas William A.
Estell David A.
Lee Sang-Kyu
Power Scott D.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Danisco Us Inc.
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