Variant proteins and polypeptides possessing enhanced...

Details Variant proteins and polypeptides possessing enhanced... Variant proteins and polypeptides possessing enhanced...

C - Chemistry, Metallurgy

12

N

C12N 15/18 (2006.01) A61K 38/27 (2006.01) C07K 1/107 (2006.01) C07K 1/22 (2006.01) C07K 14/61 (2006.01) C07K 14/65 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2021722

The present invention is directed at variant proteins and polypeptides having an enhanced affinity, i.e., greater binding strength, for immobilized-metal affinity resins and resides in engineering one or more specific metal-chelating amino acid sequences into a protein or polypeptide, the specific sequence depend- ing on the metal-binding amino acids utilized and the secondary structure associated with the portion of the protein or polypeptide to include such sequence.

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