Variants of adp-glucose pyrophosphorylase affecting...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01) C12P 19/04 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2468727

The subject invention concerns chimeric AGP subunit proteins and polynucleotides that encode the chimeric proteins. The subject invention provides for mutant AGP enzymes comprising a chimeric subunit of the invention that are less sensitive to inorganic phosphate than wild type AGP enzymes. In one embodiment, the AGP subunit is a small subunit of a plant AGP enzyme. The subject invention also concerns plants comprising a polynucleotide encoding a chimeric AGP subunit protein of the invention. The subject invention also concerns methods for producing a plant comprising a polynucleotide of the present invention. Plants produced according to the invention comprise AGP enzymes that are less sensitive to inorganic phosphate than wild type AGP enzyme.

L'invention concerne des protéines de sous-unité AGP chimérique et des polynucléotides codant pour les protéines chimériques. La présente invention concerne également des enzymes AGP mutantes comprenant une sous-unité chimérique de l'invention, moins sensibles au phosphate inorganique que les enzymes AGP de type sauvage. Dans un mode de réalisation, la sous-unité AGP est une petite sous-unité d'une enzyme AGP végétale. L'invention concerne en outre des plantes comprenant un polynucléotide codant pour une protéine de sous-unité AGP chimérique de l'invention. L'invention concerne de plus des méthodes de production d'une plante comprenant un polynucléotide de la présente invention. Les plantes produites selon l'invention comprennent des enzymes AGP moins sensibles au phosphate inorganique que l'enzyme AGP de type sauvage.

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