C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/28 (2006.01)
Patent
CA 2689635
Variants of Bacillus sp. no. 707 alpha amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
L'invention concerne des variants de l'alpha-amylase de Bacillus sp. no. 707 qui sont produits avec un meilleur rendement et de manière plus économique. Pour obtenir des rendements de fermentation plus élevés, on introduit des variations d'acides aminés qui favorisent la solubilité du variant dans un bouillon de fermentation. Une meilleure solubilité permet à plus d'enzymes de rester dans la solution après expression dans une cellule hôte. Ceci augmente ensuite le rendement avec lequel l'enzyme du variant exprimé peut être récupéré dans le bouillon de fermentation.
Aehle Wolfgang
Amin Neelam S.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Danisco Us Inc.
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