Variants of humanized anti-carcinoma monoclonal antibody cc49

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) A61K 39/395 (2006.01) A61K 51/10 (2006.01) A61P 35/00 (2006.01) C07K 16/30 (2006.01) C07K 16/46 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) G01N 33/574 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2348698

The invention is directed towards mouse-human chimeric variants of CC49 monoclonal antibodies with minimal murine content. A first aspect of the invention provides CDR variants of humanized monoclonal antibody (HuCC49) in which less than all six (three heavy chain and three light chain) Complementarity Determining Regions (CDRs) of CC49 are present. A second aspect of the invention provides SDR variants of humanized monoclonal antibody (HuCC49) in which only Specificity Determining Regions (SDRs) of at least one CDR from CC49 are present. The invention is also directed towards biotechnological methods of making the variants and therapeutic methods of using the variants.

La présente invention concerne des variants chimériques murins-humains d'anticorps monoclonaux CC49 ayant une teneur minimale en matière murine. Un premier aspect de l'invention concerne des variants CDR de l'anticorps monoclonal de type humain (HuCC49) dans lesquels moins de la totalité des six régions hypervariables (CDR) (trois chaînes lourdes et trois chaînes légères) de CC49 sont présentes. Un deuxième aspect de l'invention se rapporte à des variants SDR de l'anticorps monoclonal de type humain (HuCC49) dans lesquels seules les régions déterminant la spécificité (SDR) d'au moins une CDR provenant de CC49 sont présentes. Cette invention concerne également des procédés biotechnologiques de production des variants et des procédés thérapeutiques d'utilisation de ces mêmes variants.

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