Variation of recombinant expression titres by optimising...

Details Variation of recombinant expression titres by optimising... Variation of recombinant expression titres by optimising...

C - Chemistry, Metallurgy

12

N

C12N 15/67 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2718388

The present invention provides a method for optimising the ribosome binding site of a promoter for the expression of a gene encoding a polypeptide of interest, placed under the control of said promoter. The invention also relates to a vector containing such optimised promoters, a prokaryotic host cell transformed by said vector, as well as a method for producing a recombinant protein of interest.

La présente invention porte sur un procédé pour optimiser le site de liaison ribosomique d'un promoteur pour l'expression d'un gène codant pour un polypeptide d'intérêt, placé sous le contrôle dudit promoteur. L'invention porte également sur un vecteur contenant de tels promoteurs optimisés, sur une cellule hôte procaryote transformée par ledit vecteur, ainsi que sur un procédé de production d'une protéine recombinée d'intérêt.

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