Vascular permeability factor

Details Vascular permeability factor Vascular permeability factor

C - Chemistry, Metallurgy

12

P

167/104, 167/139

C12P 21/00 (2006.01) C07K 14/52 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1218301

- 1 - Abstract of the Disclosure A purified vascular permeability protein factor and method of manufacture, wherein the factor has the following characteristics: a) in an aqueous solution (.01M Na3PO4, pH 7) whose concentration of NaCl is varied linearly, the factor is eluted from a heparin-Sepharose chromatography column in a peak centered at 0.4M NaCl, b) in an aqueous solution of Na3PO4 (pH 7.0) whose concentration is varied linearly, the factor is eluted from a hydroxylapatite column in a peak centered at 0.25M Na3PO4; c) when subjected to SDS gel electrophoresis in a 7.5% polyacrylamide slab gel (0.375M tris-HCl (pH 8.8), 0.1% SDS) at 35 milliamps and 4°C, the factor is localized to a region corresponding to a molecular weight between 34,000 and 45,000 daltons.

441594

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