C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C07K 14/62 (2006.01) C12N 1/19 (2006.01) C12N 15/62 (2006.01) C12N 15/67 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01)
Patent
CA 2241367
The present invention relates to a DNA construct comprising a DNA sequence encoding N-terminally extended protein polypeptides, vectors carrying such DNA fragments and yeast cells transformed with the vectors, as well as a process of producing heterologous proteins in eukaryotic cells including yeast. The DNA construct encodes the following structure: signal peptide-leader peptide-EEAEPK-polypeptide.
ADN de synthèse comportant une séquence d'ADN codant des polypeptides protéiques à prolongement N-terminal, vecteurs portant de tels fragments d'ADN, cellules de levure transformées à l'aide de ces vecteurs, et procédé de production de protéines hétérologues dans des cellules eucaryotes, dont la levure. Ledit produit de synthèse de l'ADN code la structure suivante: peptide signal-peptide initial-EEAEPK-polypeptide.
Balschmidt Per
Kjeldsen Thomas Borglum
Pettersson Annette Frost
Mccarthy Tetrault Llp
Novo Nordisk A/s
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