Vector for polypeptides in bacilli

Details Vector for polypeptides in bacilli Vector for polypeptides in bacilli

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/75 (2006.01) C07K 14/31 (2006.01) C12N 1/21 (2006.01) C12N 9/54 (2006.01) C12N 15/57 (2006.01)

Patent

CA 1311429

ABSTRACT OF THE DISCLOSURE A replicable plasmidic expression vector, capable of high levels of expression and secretion of polypeptides in a bacillus is disclosed. The vector contains a DNA sequence comprising the promoter and regulatory regions which control expression and secretion of proteases in a bacillus operably liked to a DNA sequence encoding the amino acid sequence of a polypeptide. The expression vector is particularly useful in the production of B. amyloliquefaciens proteases in B. subtilis. The method facilitates the isolation of proteases from various bacilli.

457677

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