Vector for the production of transplastomic angiosperm plants

Details Vector for the production of transplastomic angiosperm plants Vector for the production of transplastomic angiosperm plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2496913

The invention relates to a vector for the production of transplastomic angiosperm plants. More specifically, the invention relates to a DNA vector which enables the stable insertion and expression of heterologous genes in the genomes of cell plastids from any angiosperm plant. In the aforementioned vector, the genes to be introduced can be inserted in multiple cloning sites which are located in an artificial intergenic region which is obtained by combining the rbcL and atpB operons from plants of different groups (dicotyledons or monocotyledons). The expression cassette is integrated into the genome by means of recombination between the rbcL and atpB border sequences of the vector with the corresponding homologous regions of the plastid genome. More than one gene of interest can be expressed from the rbcL promoter adjacent to the border region which codes for the atpB gene.

L'invention concerne un vecteur d'ADN permettant l'insertion stable de l'expression de gènes hétérologues dans les génomes de plastides de cellules d'une plante angiosperme quelconque. Dans ce vecteur, les gènes à introduire peuvent être insérés dans de multiples sites de clonage localisés dans une région intergénique artificielle que l'on obtient avec la combinaison des opérons atpB et rbcL appartenant à des plantes de types différentes (dicotylédones ou monocotilédones). La cassette d'expression est intégrée au génome au moyen de la recombinaison entre les séquences sauvages atpB et rbcL du vecteur, présentant les régions homologues correspondantes du génome plastidique. A partir du promoteur rbcL adjacent à la région sauvage codant le gène atpB on peut exprimer plus d'un gène d'intérêt.

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