Vector-mediated genomic insertion and expression of dna in bcg

Details Vector-mediated genomic insertion and expression of dna in bcg Vector-mediated genomic insertion and expression of dna in bcg

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) A61K 39/00 (2006.01) C07K 14/16 (2006.01) C12N 1/21 (2006.01) C12N 15/49 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2063414

2063414 9015873 PCTABS00003 Recombinant integration vectors useful for introduction of DNA of interest into mycobacteria are disclosed. The integration vector includes: 1) DNA sequences homologous to sequences present in the mycobacterial genome; 2) DNA encoding a polypeptide or protein of interest; 3) DNA encoding a selectable marker of mycobacterial origin; and 4) DNA encoding a regulated mycobacterial promoter region. The present invention also relates to recombinant mycobacteria useful for administration to mammalian hosts for purposes of immunization.

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